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. 2017 Mar 22;7:91. doi: 10.3389/fcimb.2017.00091

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Copyright © 2017 Li, Sheng, Wang, Yang, Liang, Huang, Liu, Li, Yang, Yang, Jia, Xie, Wang, Hao, Du, Xu, Zhou, Li, Sun, Tong, Li, Qiu and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of both HBV antigen expression and HBV replication by CRISPR-Cas9 in A64 cells after transfection. (A) DNA extracted from A64 cells transfected with gRNA-91, gRNA-69, gRNA-65, gRNA-62, and gRNA-60 was analyzed by a T7EI assay. Predicted sizes of uncut and cut bands are indicated. (B–D) Inhibition of HBsAg, HBV DNA, and HBeAg in cell culture supernatants at the indicated time points after transfection of A64 cells with gRNA-91, gRNA-69, gRNA-65, gRNA-62, and gRNA-60.