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Sanger sequencing of the gRNA-69/Cas9 target region both in the HBV-excised cell line 69-7 (upper half) and two ends of the full-length integrated HBV DNA in the stable HBV cell line A64 (lower half). The specific residual sequence with a three-nucleotide “GAA” deletion at the gRNA-69/Cas9 cleavage region in HBV-excised cell line 69-7 was used to distinguish it from the stable HBV cell line. The extra “GAA” deletion was marked by a red line in the stable HBV cell line A64.
