Figure 5.

Multiplication of neutrophil extracellular traps (NETs)-trapped bacteria. Neutrophils were stimulated with phorbol 12-myristate 13-acetate for NETs production (NETs-positive cells), and a portion of the cells were then treated with DNase I to degrade NETs (NETs-negative cells). NETs-positive and NETs-negative cells were incubated with Pseudomonas fluorescens (A), Vibrio harveyi (B), and Edwardsiella tarda (C) for different hours, and bacterial numbers were determined by plate count. The experiment was performed three times, and the results are shown as means ± SEM. **P < 0.01.