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. 2017 Mar 22;8:320. doi: 10.3389/fimmu.2017.00320

Figure 3.

Figure 3

Overexpression of Rik does not influence CD8+ T cell activation in vitro. (A) Expression of Rik after lentiviral transduction. N: naïve CD8+ T cells. A: agonistic antibody-stimulated CD8+ T cells. Ctrl: no transduction. L-S: transduction with lentiviruses containing scramble sequence. L-R: transduction with lentiviruses containing Rik sequence. This is a representative image of two independent experiments. (B) CFSE dilution in lentivirus-transduced CD8+ T cells. Left panel: representative histograms. Right panel: statistics of mean fluorescent intensity. (C,D) Intracellular staining of TNF-α (C), granzyme B and perforin (D) in lentivirus-transduced CD8+ T cells. Numbers in the plots are proportions of gated cell populations. N: naïve CD8+ T cells. A: agonistic antibody-stimulated CD8+ T cells. L-S: transduction with lentiviruses containing scramble sequence. L-R: transduction with lentiviruses containing Rik sequence. (E) Statistical analysis of the proportions of CD8+ T cells expressing TNF-α, granzyme B, and perforin. N = 5 per group. (F) CD8+ T cell apoptosis after stimulation and lentiviral transduction. A-V, Annexin V; PI, propidium iodide. This is a representative image of two independent experiments.