Figure 4.

PR8 infection induces upregulation of VCAM-1 expression on the lung vascular endothelium. Lungs from PR8-infected and PBS control mice were collected 6 days post-infection. Sections of lung tissue were stained with rat anti-mouse VCAM-1 antibody (red) and DAPI (blue). (A) Representative immunofluorescence staining of mouse lung tissue, original magnification 10×. (B) Quantification of VCAM-1 expression in lung tissue sections. The graphs show the quantification of VCAM-1 intensity/field from 5 sections per mouse. The data are pooled from 2 independent experiments with 7–8 individual mice per group. The bars show mean ± SEM (***P < 0.001).