Table 2.

List of primers used in this study.

DNA region	Primer names	Sequence 5′–3′	Reference	Thermocycling protocol
ITS	18S-1830F 26S-25R	5′-AACAAGGTTTCCGTAGGTGA-3′ 5′-TATGCTTAAAYTCAGCGGGT-3′	Nickrent et al., 1994	Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 57°C for 20 s, extension at 72°C for 45 s, and a final extension at 72°C for 7 min
trnL-F	cF fR	5′-CGAAATCGGTAGACGCTACTACG-3′ 5′-ATTTGAACTGGTGACACGAG-3′	Taberlet et al., 1991	Activation of the polymerase at 94°C for 3 min followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min
rps16	rpsF rpsR2	5′-GTGGTAGAAAGCAACGTGCGACTT-3′ 5′-TCGGGATCGAACATCAATTGCAAC-3′	Oxelman et al., 1997	Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and a final extension at 72°C for 7 min
trnK-matK-trnK	matK1198f	5′-CTGTGTTAGATATACNAATACCCC-3′	Andersson and Antonelli, 2005	Activation of the polymerase at 94°C for 4 min followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 2.5 min, and a final extension at 72°C for 7 min
	matk2053r	5′-TTAGCRCAAGAYAGTCGAAGTA-3′
ndhF	1320f	5′GGGATTAACYGCATTTTATATGTTTCG-3′	Rydin et al., 2008	Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min
ndhF	1000r	5′-CCTAGAGCTAGCATCATATAACCC-3′