Table 2.
DNA region | Primer names | Sequence 5′–3′ | Reference | Thermocycling protocol |
---|---|---|---|---|
ITS | 18S-1830F 26S-25R |
5′-AACAAGGTTTCCGTAGGTGA-3′ 5′-TATGCTTAAAYTCAGCGGGT-3′ |
Nickrent et al., 1994 | Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 57°C for 20 s, extension at 72°C for 45 s, and a final extension at 72°C for 7 min |
trnL-F | cF fR |
5′-CGAAATCGGTAGACGCTACTACG-3′ 5′-ATTTGAACTGGTGACACGAG-3′ |
Taberlet et al., 1991 | Activation of the polymerase at 94°C for 3 min followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min |
rps16 | rpsF rpsR2 |
5′-GTGGTAGAAAGCAACGTGCGACTT-3′ 5′-TCGGGATCGAACATCAATTGCAAC-3′ |
Oxelman et al., 1997 | Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and a final extension at 72°C for 7 min |
trnK-matK-trnK | matK1198f | 5′-CTGTGTTAGATATACNAATACCCC-3′ | Andersson and Antonelli, 2005 | Activation of the polymerase at 94°C for 4 min followed by 35 cycles of denaturation at 94°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 2.5 min, and a final extension at 72°C for 7 min |
matk2053r | 5′-TTAGCRCAAGAYAGTCGAAGTA-3′ | |||
ndhF | 1320f | 5′GGGATTAACYGCATTTTATATGTTTCG-3′ | Rydin et al., 2008 | Activation of the polymerase at 95°C for 3 min followed by 35 cycles of denaturation at 95°C for 1 min, annealing at 50°C for 1 min, extension at 72°C for 1 min, and a final extension at 72°C for 7 min |
ndhF | 1000r | 5′-CCTAGAGCTAGCATCATATAACCC-3′ |