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. 2017 Mar 22;8:283. doi: 10.3389/fimmu.2017.00283

Figure 4.

Figure 4

CD4+CD62L+ non-effector T cell (Teff) subsets improve the immunosuppressive effects of CD4+CD25high Tregs in an age-dependent manner. (A) Splenocytes were harvested from young and old mice, enriched for CD4+ T cells by magnetic separation, stained for CD4, CD62L, and CD25, and subsequently sorted into a CD4+CD62L−CD25− Teff subset (Aa), a CD4+CD62L+CD25– non-Teff subset (Ab) or CD4+CD25high Treg cells as shown in Figure 3A. The sorted CD4+CD62L−CD25− Teff subsets were cocultured for 48 h with CD4+CD25high Treg cells at a2:1 ratio, with or without sorted CD4+CD62L+CD25− non-Teff subsets, and then cytokine production was measured in the supernatant by ELISA. (B,D,F) Cytokine secretion levels. (C,E,G) Percent cytokine secretion in the presence of CD4+CD62L+CD25− non-Teffs. Data are shown as means ± SEM of eight mice per group, pooled from two independent experiments (each with four mice per group). p values were calculated by Student’s t-test; *p < 0.05.