Table 1.

Summary of behavioral assays.

Assay	Resemblance to core feature of ASDs	Quantitative measurements
Three chamber test	Social communication deficits	Phase 1: “Sociability” - preference for social stimulus (stranger mouse) over an inanimate object Phase 2: “Social novelty” - preference for an unfamiliar mouse over the stranger mouse used in the sociability phase
Habituation/dishabituation	Social communication deficits	Time spent interacting with the same mouse during the trial period over several days and time spent with a novel mouse on the last trial
Partition test	Social communication deficits	Time spent near a barrier that separates the test mouse from a stranger mouse
Direct social interaction tests	Social communication deficits	Duration, type (e.g. aggressive), and amount of social interactions
Ultrasonic vocalizations	Social communication deficits	Number, duration, and complexity of vocalizations
Nest-building	Social communication deficits	Amount of nesting material used, size of nest, or numerical scoring system
Self-grooming	Repetitive behavior	Time spent grooming or number of grooming bouts
Jumping	Repetitive behavior	Time spent jumping or number of jumping bouts
Digging	Repetitive behavior	Time spent digging or number of digging bouts
Hole board test	Repetitive behavior	Number of total hole-pokes or number of consecutive (2+) pokes in the same hole
Marble-burying test	Repetitive behavior	Number of marbles buried