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. Author manuscript; available in PMC: 2017 Mar 22.
Published in final edited form as: Sci Transl Med. 2015 Apr 1;7(281):281ra45. doi: 10.1126/scitranslmed.aaa5171

Figure 6. Pediatric patients with heart disease show decreased cardiomyocyte cell cycle activity.

Figure 6

Cardiomyocytes from patients were isolated, stained, and analyzed by flow cytometry. (A) Isolated human cardiomyoctes were intact as evidenced by staining with antibodies against pan-cadherin and α-actinin. (B) Representative double marker plot of a 3 month old patient showing flow cytometry analysis of cardiomyocyte cell cycle activity using cardiomyocyte (α-actinin) and cell cycle markers (H3P). (C) Summary graph shows that patients with heart disease exhibit decreased cycling compared to age-matched controls without heart disease. Numbers of patients per data point are indicated in red, no heart disease were 1 patient per data point. Circles represent RV and triangles LV samples. Scale bar: 50 μm.