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. 2017 Mar 22;3(1):00107-2016. doi: 10.1183/23120541.00107-2016

FIGURE 1.

FIGURE 1

Effects of BAs on BEAS-2B cell viability. BEAS-2B cell viability following stimulation with a) lithocholic, b) deoxycholic, c) cholic and d) chenodeoxycholic acids at varying concentrations. Viability is measured as a percentage of vehicle control after 48 h exposure. Data are presented as mean±sem. Negative controls were measured by fixing the cells in methanol before adding the TiterBlue and MTT reagent (n=6). C: control; CTB: CellTiter-Blue assay; MTT: thiazolyl blue tetrazolium bromide. **: p=0.01; ****: p<0.0001 versus controls.