Table 4.
Stability of quercetin, luteolin, and apigenin in rat plasma (n = 6).
| Compounds | Spiked conc. (μg/ml) | Short-term stability (24 h at room temperature) |
Long-term stability (30 days at −20°C) |
Freeze-thaw stability (3 cycles) |
|||
|---|---|---|---|---|---|---|---|
| Found conc. (μg/ml) | RSD (%) | Found conc. (μg/ml) | RSD (%) | Found conc. (μg/ml) | RSD (%) | ||
| Quercetin | 0.57 | 0.63 ± 0.01 | 1.30 | 0.62 ± 0.03 | 5.16 | 0.63 ± 0.01 | 2.08 |
| 2.84 | 3.09 ± 0.18 | 5.68 | 3.10 ± 0.16 | 5.17 | 3.09 ± 0.09 | 2.78 | |
| 9.09 | 9.15 ± 0.69 | 7.52 | 9.59 ± 0.53 | 5.52 | 9.24 ± 0.55 | 5.97 | |
| Luteolin | 0.56 | 0.62 ± 0.01 | 1.21 | 0.61 ± 0.03 | 5.52 | 0.62 ± 0.02 | 3.01 |
| 2.80 | 2.94 ± 0.14 | 4.65 | 2.92 ± 0.15 | 5.03 | 2.91 ± 0.15 | 5.23 | |
| 8.96 | 8.96 ± 0.76 | 8.53 | 8.99 ± 0.65 | 7.28 | 8.56 ± 0.54 | 6.33 | |
| Apigenin | 0.53 | 0.50 ± 0.04 | 8.36 | 0.46 ± 0.04 | 8.47 | 0.48 ± 0.02 | 3.21 |
| 2.65 | 2.64 ± 0.16 | 6.14 | 2.61 ± 0.13 | 5.07 | 2.60 ± 0.14 | 5.31 | |
| 8.48 | 8.31 ± 0.72 | 8.61 | 8.27 ± 0.62 | 7.46 | 7.87 ± 0.50 | 6.39 | |