Figure 4.

Effect of andrographolide and/or brusatol on Aβ₄₂ expression in BV-2 cells. BV-2 cells were transfected with Aβ₄₂ plasmids for 12 h. The medium was discarded and replaced with phenol red-free medium in the absence or presence of 100 nM brusatol. After 2 h, the medium was replaced with fresh medium containing 1, 5, or 10 μM of andrographolide. The abbreviation of andrographolide, ANDRO, was used. (a) The microglial clearance of Aβ₄₂ was visualized by fluorescence microscope imaging. (b) The relative fluorescence intensity of Aβ₄₂ was quantified by Image J software. The data are presented as the means ± SE of triplicates. The significance presented as ^∗∗∗p < 0.001 compared with control group and ^##p < 0.01 and ^###p < 0.001 compared with the Aβ₄₂-transfected group. (c) The secreted NO levels in the medium of BV-2 cells were measured using the Griess method. The data are presented as the means ± SE of triplicates. The significance presented as ^∗∗∗p < 0.001 compared with control group and ^###p < 0.001 compared with the Aβ₄₂-transfected group.