Figure 6.

Effect of andrographolide on the secretion inflammatory cytokines, PGE₂ and NO. BV-2 cells were seeded onto culture dishes and transfected with Aβ₄₂ plasmids for 12 h. Subsequently, the medium was replaced with fresh phenol red-free medium containing various concentrations of andrographolide for 24 h. The conditioned medium was collected and used to analyze the secretion of IL-1β, IL-6, PGE₂, and NO. The abbreviation of andrographolide, ANDRO, was used. The data are presented as the means ± SE of triplicates. The significance presented as ^∗p < 0.05 and ^∗∗∗p < 0.001 compared with control group and ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001 compared with the Aβ₄₂-transfected group.