Fig. 1.
Nucleic acid extraction and downstream application.
A. Neutral salting out (DNA extraction).
B. Acidic salting out (RNA extraction).
C. GoldView™ Nucleic Acid Stained 1.5% Agarose gel demonstrating the integrity of total DNA extracted from Eimeria tenella.
D. GoldView™ Nucleic Acid Stained 1.5% Agarose gel of total RNA of E. tenella.
E. Standard PCR amplification of MICII of E. tenella.
F. GoldView™ Nucleic Acid Stained 1.5% Agarose gel of EtMIC-2 RT-PCR amplified product of E. tenella using Finnzymes phusion™ High-Fidelity DNA Polymerase.