Table 2.

The design point combinations and the corresponding experimental responses for AMD 1, AMD 2 and AMD 3.

Run	Variable	Levels	Responses in	Yield (%)
	Temperature (°C)	pH	Time (Min.)	AMD1	AMD2	AMD3
1	50	5	45	58.2	50.4	54
2	50	8	30	83	80.2	79.5
3	30	5	30	40	37	38.2
4	50	11	45	76.5	72.6	73.2
5	50	8	30	83.4	79.9	79.5
6	30	8	15	63.9	55	59
7	70	11	30	70.1	68	63
8	50	5	15	58	52.7	51.5
9	50	8	30	83.2	80.5	80.4
10	50	8	30	83	80.2	80.3
11	70	5	30	51.2	46.5	49
12	30	11	30	59.6	53.5	57
13	30	8	45	63	59.3	61.2
14	70	8	45	74.6	69.2	70
15	50	11	15	76.8	70	69
16	70	8	15	74	70	67.4
17	50	8	30	82.8	80.5	80.8

AMD 1: 2-butyramidoacetic acid.

AMD 2: 2-benzamidoacetic acid.

AMD3: 2,2′((carboxymethyl)amino)-2-oxoethyl)-2-hydroxysuccinyl)bis(azanediyl))diacetic acid.