Fig. 2.

Influence of pore formation and pore size for a freeze-drying approach with protein based hydrogels frozen at different temperatures. (A) Size and distribution of pores within the protein hydrogels matrix for hydrogels frozen in liquid nitrogen and at −20 °C (B)(C) Hydrogels were frozen and in liquid nitrogen (B) and at −20 °C (C) followed by subsequent freeze-drying, stained with rhodamine B and investigated via confocal laser scanning microscopy. Scale bar 50 μm.