Figure 3.
(A) The effect of using real-time signal. (A) a set (assay) of compartments with geometrical sequence of modulation factor divi. Larger compartments yield positive digital signals while the other yield negative digital signals. (B) Digital signals from compartments can be used to determine the probability density function of the concentration of the analyte that caused such state of the assay. (C) If the digital measurement is accompanied with analogue measurement, sigmoidal functions of probability for positive compartments can be shifted towards the real value of concentration. Hence, they all contribute to the estimation of initial concentration and provide for higher precision. (D) The new probability density function of concentration can be calculated, which is narrower than the function based solely on digital measurement, and therefore provides higher precision (lower relative standard deviation) of the estimate of concentration.