. 2017 Mar 22;11:73. doi: 10.3389/fncel.2017.00073

Table 1.

Protective effect of A. reticulata and Z. jujuba plant extracts and fractions against H₂O₂ intoxicated SHSY5Y cell lines.

Group no.	Treatment	Concentration	% Cell viability	LDH (U/L)	TBARS (nmol/mg protein)
Group-I (Control)	Normal	No treatment	98.5 ± 1.84	84.6 ± 0.94	1.84 ± 0.08
	DMSO	0.25% V/V	97.8 ± 2.12	85.7 ± 1.03	1.87 ± 0.07
	ARBME	200 μg/ml	96.8 ± 1.73	86.2 ± 0.82	1.86 ± 0.07
	ARBHF	200 μg/ml	96.5 ± 1.66	85.4 ± 0.97	1.78 ± 0.08
	ARBCF	200 μg/ml	96.7 ± 2.03	86.6 ± 1.12	1.81 ± 0.07
	ARBEAF	200 μg/ml	96.2 ± 1.85	85.2 ± 1.07	1.89 ± 0.09
	ARBWF	200 μg/ml	96.4 ± 1.92	86.8 ± 0.96	1.86 ± 0.08
	ARBMR	200 μg/ml	96.7 ± 1.64	85.6 ± 0.81	1.82 ± 0.07
	ZJME	200 μg/ml	97.1 ± 2.16	85.2 ± 0.88	1.79 ± 0.08
	ZJHF	200 μg/ml	96.7 ± 1.78	85.6 ± 0.96	1.88 ± 0.09
	ZJCF	200 μg/ml	96.4 ± 1.46	86.1 ± 1.14	1.85 ± 0.08
	ZJEAF	200 μg/ml	97.3 ± 2.07	85.7 ± 0.86	1.87 ± 0.09
	ZJWF	200 μg/ml	96.8 ± 1.62	86.8 ± 0.95	1.83 ± 0.07
	ZJMR	200 μg/ml	96.6 ± 2.15	85.8 ± 1.12	1.78 ± 0.08
Group-II	H₂O₂ control	100 μM	38.7 ± 2.73^∧	157.9 ± 4.26^∧	4.28 ± 0.24^∧
Group-III	ARBME + H₂O₂	50 μg/ml + 100 μM	69.2 ± 3.47^*	117.4 ± 3.16^*	2.97 ± 0.17^*
		100 μg/ml + 100 μM	76.4 ± 3.82^*	106.8 ± 2.68^*	2.53 ± 0.15^*
		200 μg/ml + 100 μM	84.6 ± 3.25^*^#	94.2 ± 2.19^*^#	2.18 ± 0.11^*^#
	ARBHF + H₂O₂	50 μg/ml + 100 μM	41.2 ± 2.81	152.4 ± 3.62	4.12 ± 0.21
		100 μg/ml + 100 μM	43.6 ± 2.64	151.7 ± 3.85	4.06 ± 0.23
		200 μg/ml + 100 μM	43.9 ± 3.54	150.5 ± 4.16	4.04 ± 0.21
	ARBCF + H₂O₂	50 μg/ml + 100 μM	39.5 ± 3.17	157.2 ± 4.07	4.15 ± 0.26
		100 μg/ml + 100 μM	40.8 ± 2.62	155.2 ± 3.42	4.11 ± 0.23
		200 μg/ml + 100 μM	41.7 ± 2.86	152.8 ± 4.21	4.08 ± 0.19
	ARBEAF + H₂O₂	50 μg/ml + 100 μM	40.4 ± 3.45	156.7 ± 3.27	4.09 ± 0.22
		100 μg/ml + 100 μM	41.8 ± 2.69	153.5 ± 4.11	4.08 ± 0.25
		200 μg/ml + 100 μM	42.6 ± 3.14	152.1 ± 3.68	4.02 ± 0.19
	ARBWF + H₂O₂	50 μg/ml + 100 μM	78.6 ± 3.86^*	104.8 ± 2.59^*	2.56 ± 0.11^*
		100 μg/ml + 100 μM	86.2 ± 4.17^*	90.7 ± 2.81^*	2.21 ± 0.13^*
		200 μg/ml + 100 μM	92.1 ± 3.66^*^#	88.2 ± 2.44^*^#	2.02 ± 0.09^*^#
	ARBMR + H₂O₂	50 μg/ml + 100 μM	44.5 ± 3.92	142.6 ± 3.27	3.46 ± 0.17
		100 μg/ml + 100 μM	49.8 ± 2.73	137.8 ± 3.41	3.24 ± 0.18
		200 μg/ml + 100 μM	55.3 ± 4.14^*	131.2 ± 2.85^*	3.11 ± 0.16^*
Group-IV	ZJME + H₂O₂	50 μg/ml + 100 μM	72.8 ± 4.27^*	114.3 ± 3.62^*	2.72 ± 0.19^*
		100 μg/ml + 100 μM	79.6 ± 3.62^*	102.6 ± 3.05^*^#	2.44 ± 0.14^*
		200 μg/ml + 100 μM	88.5 ± 4.08^*^#	89.7 ± 2.43^*^#	2.07 ± 0.12^*^#
	ZJHF + H₂O₂	50 μg/ml + 100 μM	40.8 ± 3.48	155.2 ± 4.16	4.02 ± 0.19
		100 μg/ml + 100 μM	41.2 ± 2.86	152.8 ± 3.45	3.97 ± 0.21
		200 μg/ml + 100 μM	41.8 ± 3.16	151.9 ± 4.21	3.92 ± 0.18
	ZJCF + H₂O₂	50 μg/ml + 100 μM	39.2 ± 2.44	158.2 ± 3.86	4.07 ± 0.23
		100 μg/ml + 100 μM	40.3 ± 3.24	156.4 ± 4.08	4.01 ± 0.17
		200 μg/ml + 100 μM	41.1 ± 2.61	154.3 ± 3.58	3.95 ± 0.19
	ZJEAF + H₂O₂	50 μg/ml + 100 μM	43.7 ± 2.54	149.6 ± 2.96	4.03 ± 0.19
		100 μg/ml + 100 μM	44.2 ± 3.16	148.2 ± 3.38	3.94 ± 0.21
		200 μg/ml + 100 μM	44.9 ± 3.32	147.1 ± 3.02	3.88 ± 0.21
	ZJWF + H₂O₂	50 μg/ml + 100 μM	80.7 ± 4.38^*	101.7 ± 2.47^*	2.41 ± 0.14^*
		100 μg/ml + 100 μM	89.6 ± 4.72^*	88.1 ± 2.13^*^#	2.08 ± 0.11^*
		200 μg/ml + 100 μM	94.7 ± 5.16^*^#	86.8 ± 2.26^*^#	1.91 ± 0.08^*^#
	ZJMR + H₂O₂	50 μg/ml + 100 μM	42.4 ± 4.11	150.6 ± 3.11	3.73 ± 0.22
		100 μg/ml + 100 μM	43.5 ± 3.78	149.2 ± 2.94	3.59 ± 0.17
		200 μg/ml + 100 μM	44.7 ± 3.21	148.4 ± 3.01	3.45 ± 0.19

All the results were presented in mean ± S.D (n = 5).

^∧

p ≤ 0.05 in comparison of toxin treated control group (Group-II) with normal control.

p ≤ 0.05 in comparison of group-III and IV with group-II.

p ≤ 0.05 in comparison between the different doses of same treatment. ARBME: A. reticulata bark methanol extract; ARBHF, A. reticulata bark hexane fraction; ARBCF, A. reticulata bark chloroform fraction; ARBEAF, A. reticulata bark ethyl acetate fraction; ARBWF, A. reticulata bark water fraction; ARBMR, A. reticulata bark methanol residue. ZJME, Z. jujuba root bark methanol extract; ZJHF, Z. jujuba root bark hexane fraction; ZJCF, Z. jujuba root bark chloroform fraction; ZJEAF, Z. jujuba root bark ethyl acetate fraction; ZJWF, Z. jujuba root bark water fraction; ZJMR, Z. jujuba root bark methanol residue.