Table 4.
Effect of drug treatment on sciatic nerve lipid peroxidation and antioxidant enzyme levels.
| Group No. | Group | SOD (U/ml) | CAT (nmol/min/ml) | GSH (nm/mg protein) | TBARS (nmol/g tissue) |
|---|---|---|---|---|---|
| I | Normal animal (Sham control) | 11.8 ± 1.9 | 6.8 ± 0.7 | 9.7 ± 0.92 | 143.8 ± 7.4 |
| II | Diabetic control | 3.2 ± 0.9 | 1.8 ± 0.4∧ | 2.4 ± 0.47∧ | 317.6 ± 14.8∧ |
| III | Diabetic animals + Insulin (5 IU/kg) | 5.1 ± 1.2* | 3.2 ± 0.6 | 4.2 ± 0.64* | 266.4 ± 16.3 |
| IV | Diabetic animals+ Pregabalin (100 mg/kg)+ Insulin (5 IU/kg) | 7.6 ± 1.7*$ | 3.9 ± 1.1* | 5.9 ± 0.75*$ | 248.7 ± 13.4* |
| V | Diabetic animals+ ARBME (200 mg/kg)+ Insulin (5 IU/kg) | 6.3 ± 1.1* | 4.1 ± 0.8* | 5.1 ± 0.68* | 241.5 ± 12.7* |
| VI | Diabetic animals+ ARBME (400 mg/kg)+ Insulin (5 IU/kg) | 7.7 ± 1.4*$ | 4.6 ± 0.7* | 6.5 ± 0.73*$ | 219.6 ± 14.6*$# |
| VII | Diabetic animals+ ARBWF (50 mg/kg) Insulin+ (5 IU/kg) | 7.1 ± 1.7* | 4.4 ± 0.9* | 6.2 ± 0.59*$ | 227.5 ± 15.2*$ |
| VIII | Diabetic animals+ ARBWF (100 mg/kg)+ Insulin (5 IU/kg) | 8.4 ± 1.5*$ | 5.1 ± 1.1*$ | 7.1 ± 0.84*$ | 204.9 ± 12.3*$ |
| IX | Diabetic animals+ ZJME (200 mg/kg)+ Insulin (5 IU/kg) | 6.5 ± 1.6* | 4.3 ± 0.8* | 5.3 ± 0.64* | 233.1 ± 16.8*$ |
| X | Diabetic animals+ ZJME (400 mg/kg)+ Insulin (5 IU/kg) | 7.9 ± 1.8*$ | 4.8 ± 0.9* | 6.9 ± 0.77*$# | 211.6 ± 14.4*$# |
| XI | Diabetic animals+ ZJWF (50 mg/kg)+ Insulin (5 IU/kg) | 7.4 ± 1.4*$ | 4.7 ± 1.3* | 6.5 ± 0.71*$ | 220.4 ± 12.3*$ |
| XII | Diabetic animals+ ZJWF (100 mg/kg)+ Insulin (5 IU/kg) | 8.7 ± 1.7*$ | 5.4 ± 1.2*$ | 7.6 ± 0.87*$ | 196.2 ± 16.6*$# |
All the results were presented in mean ± S.D (n = 6).
∧
p ≤ 0.05 in comparison of diabetic animals (Group-II) with normal animals (Group-I).
*
p ≤ 0.05 in comparison of drug treated groups (Group-III–XII) with diabetic control group (Group-II).
$
p ≤ 0.05 in comparison of drug treatment along with insulin treated groups (Group-IV to XII) with insulin alone treated group (Group-III).
#
p ≤ 0.05 in comparison between the different doses of same treatment. ARBME, A. reticulata bark methanol extract; ARBWF, A. reticulata bark water fraction; ZJME, Z. jujuba root bark methanol extract; ZJWF, Z. jujuba root bark water fraction.