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. 2017 Feb 8;7(18):10660–10667. doi: 10.1039/c6ra27684a

Table 3. Shift of melting temperature, ΔT m, of quadruplex DNA F21T in the presence of the ligands 2 and 4b–d at different LDR without and with the duplex DNA ds26 as determined by fluorimetric thermal denaturation studies.

Ligand ΔT m/°C at different LDR a
Without ds26
With ds26 b
2.5 5.0 2.5 5.0
2BF4 21.7 27.0 20.0 26.5
4b 28.2 31.6 21.3 30.7
4c 25.9 31.8 23.3 29.4
4d 28.1 32.9 25.5 31.4

aΔT m of F21T; c F21T = 0.2 μM (in oligonucleotide); KCl–LiCl–Na–cacodylate buffer (10 mM K+, 10 mM Na+, 90 mM Li+, pH 7.2); λ ex = 470 nm; λ em = 515 nm; estimated error: ±0.5 °C of the given data.

b c ds26 = 3.0 μM.