Skip to main content
. 2017 Mar 22;7:45228. doi: 10.1038/srep45228

Figure 1. Production of Huh7- and Hep3B-adapted HCVcc.

Figure 1

(A) In vitro-transcribed JFH1-AM (left panel) and JFH1-WT (right panel) RNA were electroporated into Huh7.5.1 (red line) and Hep3B/miR-122 (blue line) and infectious titers of each cell line were examined by focus forming assay. (B) Adaptive mutations of JFH1-AM and JFH1-WT in the supernatants of Huh7.5.1 and Hep3B/miR-122 cells. (C) Schematic diagram of adaptation of HCVcc to new target cells for the examination of the significance of quasispecies of HCV in infection to naive liver graft.