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. 2017 Mar 22;7:45228. doi: 10.1038/srep45228

Figure 3. Serial virus passages induce cell-specific adaptive mutations for high replicative fitness.

Figure 3

(A) Distribution of mutations in JFH1-am1, -am2, -am3 and -am4. (B) In vitro-transcribed viral RNAs of JFH1-WT (grey), -AM (black), -am1 (blue), -am2 (red), -am3 (green) and -am4 (purple) were electroporated into Hep3B/miR-122 and Huh7.5.1 cells and infectious titers in the supernatants were determined at several points by focus forming assay. (C,D) Equivalent amounts of JFH1-WT, -am2 and -am3 RNA were co-electroporated into Hep3B/miR-122 (blue line) and Huh7.5.1 cells (red line), and infectious titers (C) and the sequences at the positions of amino acids 416 and 417 (D) in the supernatants were determined at 4, 10, 15 and 20 days post-electroporation by focus forming assay and direct sequencing, respectively.