Table 1. Animal number in each group for different experiments.
NS + sham | Rb1 5 + sham | NS + I/R | Rb1 2.5 + I/R | Rb15 + I/R | Rb17.5 + I/R | Total | |
---|---|---|---|---|---|---|---|
*Myocardial blood flowand myocardial infarct size | 6 | 6 | 6 | 6 | 6 | 6 | 36 |
HE staining | 3 | 3 | 3 | 3 | 12 | ||
*Hemodynamics and ELISA | 8 | 8 | 8 | 8 | 32 | ||
RBC velocity | 6 | 6 | 6 | 6 | 24 | ||
F-actin-TUNEL double staining | 3 | 3 | 3 | 3 | 12 | ||
Ultra-structure examination | 3 | 3 | 3 | 3 | 12 | ||
Western blot assay and PCR | 4 | 4 | 4 | 4 | 16 | ||
Total | 33 | 33 | 33 | 6 | 33 | 6 | 144 |
*The two experiments were undertaken using the same set of animals. NS + sham: saline plus sham group; Rb1 5 + sham: Rb1 5 mg/kg/h plus sham group; NS + I/R: saline plus I/R group; Rb1 2.5 + I/R: treatment with Rb1 at 2.5 mg/kg/h plus I/R group; Rb1 5 + I/R: treatment with Rb1 at 5 mg/kg/h plus I/R group; Rb1 7.5 + I/R: treatment with Rb1 at 7.5 mg/kg/h plus I/R group.