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. 2017 Mar 22;7:45096. doi: 10.1038/srep45096

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The proportion of amplicons (of 50% methylated control) generated from assays with ‘no modifications’, ‘single modifications’ or double modifications. Baseline level of methylation (50%) is highlighted as ★. The number of amplicons in each assay and the corresponding primer concentration and melting temperature is as summarised in table. The [primer concentration] represents the concentration of the forward and reverse primer for every PCR assay in the multiplex pool.