Table 6. Chi-square* analysis for the segregation in pseudo-F2 progeny of common waterhemp based on the relative EPSPS gene copy number and phenotypic data.
Progeny |
EPSPS gene copy number |
21 d after glyphosate application† |
||||||
---|---|---|---|---|---|---|---|---|
Plants with 1.4 or more copies (GR) | Plants with one copy (GS) | Total | χ2 | Alive (GR) | Dead (GS) | Total | χ2 | |
F2 | 41 | 2 | 43 | 9.49 | 39 | 5 | 44 | 4.36 |
*The Chi-square goodness of fit test (df = 1, α = 0.05) is used to compare the observed segregation in F2 progeny with the expected Mendelian segregation for a single gene (3GR:1GS). The calculated χ2 > 3.84 indicates that the observed segregation does not agree with the expected segregation.
†Glyphosate was applied at 1,575 g ae ha−1 and the plant survival data were recorded.