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. 2017 Mar 22;7:44913. doi: 10.1038/srep44913

Table 6. Chi-square* analysis for the segregation in pseudo-F2 progeny of common waterhemp based on the relative EPSPS gene copy number and phenotypic data.

Progeny EPSPS gene copy number
21 d after glyphosate application
Plants with 1.4 or more copies (GR) Plants with one copy (GS) Total χ2 Alive (GR) Dead (GS) Total χ2
F2 41 2 43 9.49 39 5 44 4.36

*The Chi-square goodness of fit test (df = 1, α = 0.05) is used to compare the observed segregation in F2 progeny with the expected Mendelian segregation for a single gene (3GR:1GS). The calculated χ2   > 3.84 indicates that the observed segregation does not agree with the expected segregation.

Glyphosate was applied at 1,575 g ae ha−1 and the plant survival data were recorded.