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. 2017 Mar 22;7:45033. doi: 10.1038/srep45033

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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COS-1 cells were transfected with pTM2-FVIII expressing WT or S584T, D580H, I566T and N582D mutants and labeled with [35S]-methionine/cysteine. FVIII was immunoprecipitated with anti-FVIII beads and digested with thrombin or left untreated. Samples were separated by reducing SDS-PAGE. Indicated are FVIII and specific thrombin cleavage products of FVIII. Representative images of two independent experiments are shown.