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. 2017 Mar 22;7:45033. doi: 10.1038/srep45033

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Copyright © 2017, The Author(s)

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(A) Thirty-six hours after transfection, cell extracts were analyzed by immunoblotting with the indicated antibodies. CRT: calreticulin, CNX: calnexin. BiP intensities were densitometrically quantified and expressed as fold changes compared to WT after normalizing to the β-actin loading control (data are mean ± SEM, n = 2. *P < 0.05). (B) Thirty-six hours after transfection, cell extracts were immunoprecipitated with mouse monoclonal anti-Flag antibody or normal mouse IgG. The immunoprecipitates were analyzed by immunoblotting with the indicated antibodies. The amounts of BiP and CRT that co-immunoprecipitated with A2 domain mutants were densitometrically quantified and expressed as fold changes relative to WT (data are mean ± SEM, n = 3. *P < 0.05).