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. 2017 Mar 22;7:45058. doi: 10.1038/srep45058

Figure 1. Relation between FRET, fluorescence lifetime, and force applied to the EcadTSMod biosensor.

Figure 1

Schematic representation of the EcadTSMod biosensor. In absence of tension (top) the TSMod is not elongated, the distance between the two fluorescent proteins is the shortest, meaning that the FRET is maximum. As the relation between the FRET and donor fluorescence lifetime is inverse, in absence of tension, the donor fluorescence lifetime is low. In presence of force (bottom) the TSMod is elongated, the distance between fluorescent proteins increases, the FRET decreases and the donor fluorescence lifetime increases.