Figure 2. E-cadherin tension sensor displays a FRET decrease in living Xenopus embryo compared to tail less E-cadherin.

(a) Schematic representations of the different constructions used, showing binding regions of p120 catenin, β-catenin and indirect binding of α-catenin and actin, and their respective localization in epithelial cells of Xenopus laevis blastula (fluorescence), each field of view is composed of about 15 to 20 cells at this stage of development. The fluorescence lifetime images are presented for all constructions (fluorescence lifetime). (b) Boxplot representative of the mean fluorescence lifetime per field obtained over all acquisitions for the various constructs. Number of acquisition N_acq = 48, 105 and 44, number of independent experiment N_exp = 10, 14, 6 and number of embryos N_emb = 22, 55, 15, for respectively EcadTI, EcadTSMod and EcadTL. For each comparison, the p value is indicated. In the box plot, bold bars correspond to median value, whiskers to the value 1.5x away from the 1^st and 3^rd quartiles, and the dots to outliers.