Figure 2. Measurement of isozyme-specific TG activities in liver extracts.

Each liver extract was examined for in vitro enzymatic activities on the indicated days after BDL surgery (n = 4 mice) using the biotinylated peptides pepK5 (for TG1) and pepT26 (for TG2). Mutant peptides (pepK5QN and pepT26QN) were used as negative controls. (A) Each biotinylated peptide was incorporated into β-casein coated on the microtiter wells in the presence of the liver extracts. The amounts of the β-casein that were incorporated with the peptides were measured using peroxidase-conjugated streptavidin. (**P < 0.01) (B and C) The amount of Lys-donor substrates incorporated with biotinylated peptides on the blotting membrane was detected using peroxidase-conjugated streptavidin. The sizes of the protein mass markers are shown on the left. Arrowheads indicate the bands that increased compared with the control sample (Day 0).