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. Author manuscript; available in PMC: 2017 Jun 5.
Published in final edited form as: Nature. 2016 Dec 5;541(7635):102–106. doi: 10.1038/nature20789

Extended Data Figure 3. Effects of sfa-1 downregulation on splicing.

Extended Data Figure 3

a, uaf-2 gene expression is not affected by reduced sfa-1 levels in WT worms at day 1 of adulthood (mean ±SD, technical replicates shown). Effect of reduced sfa-1 expression on pumping rates in WT and genetic DR model eat-2(ad1116) at day 1 (b) and 4 of adulthood (c) (mean ±SD, ns p > 0.05, unpaired, two-tailed t-test, n=10 worms per condition). d, Splicing reporter pattern with sfa-1 knockdown from egg hatch, day 1, 3 and 7 old adults. e, Endogenous ret-1 exon 5 splicing pattern with age and sfa-1 RNAi in WT and DR worms by RT-PCR (day 3 vs.15, independent replicate sample set).