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. 2016 Oct 3;122(1):35–42. doi: 10.1080/03009734.2016.1228721

Figure 1.

Figure 1.

A: Schematic representation of the under-agarose migration assay. Wells are 3.5 mm wide and 2.5 mm apart. The two outer wells were loaded with 10 μL of 107 leukocytes/mL from a newborn infant or an adult (1 × 105 leukocytes/well). The central wells were loaded with IL-8 or fMLP or buffer (negative controls). By subtracting the number of neutrophils that migrated into the B-zone from the number in the A-zone, the number of neutrophils undergoing chemotaxis can be determined. B: An example of the migrating cells in the A-zone with 4× magnification, towards gradients of a chemoattractant. Note that only neutrophils have migrated into the A-zone at this time point.