Figure 1.

NeuN immunohistochemistry (A–D) and F-J B histofluorescence staining (E–H) in the hippocampal CA1 region of the vehicle-sham (A, E), vehicle-ischemia (C, G), QE-sham (B, F) and QE-ischemia (D, H) groups.

A few NeuN⁺ neurons and many F-J B⁺ cells are observed in the SP 5 days after ischemia (asterisks), whereas abundant NeuN⁺ and few F-J B⁺ pyramidalneurons are observed in the QE-ischemia group. Scale bars are 20 μm in D and H, valid for A–H. (I, J) Relative analysis as percent in the mean number of NeuN⁺ and F-J B⁺ cells in a 250 × 250 μm² (boxes) (n = 7 at each time point in each group, *P < 0.05, vs. vehicle-sham group; #P < 0.05, vs. corresponding vehicle-ischemia group). Data in I and J are exprossed as the mean ± SEM. QE: Quercetin; NeuN: neuronal nuclear antigen; F-J B: Fluoro-Jade B; SP: stratum pyramidale; SO: stratum oriens; SR: stratum radiatum; d: days.