Figure 2.

SOD1 and SOD2 immunoreactivities in the hyippocampal CA1 region of the vehicle-sham (A, H), vehicle-ischemia (C, E, J, L), QE-sham (B, I) and QE-ischemia (D, F, K, M) groups.

At 2 days post-ischemia, SOD1 and SOD2 immunoreactivities are decreased in the hippocampal CA1 pyramidal neurons, and at 5 days post-ischemia, SOD1 and SOD2 immunoreactivities in the CA1 pyramidal neurons are very weak (asterisk); however, strong SOD1 and SOD2 immunoreactivities are newly shown in non-pyramidal cells (arrows). In the QE-sham group, SOD1 and SOD2 immunoreactivities are significantly increased in the hippocampal CA1 pyramidal neurons compared with those in the vehicle-sham group. In the QE-ischemia group, SOD1 and SOD2 immunoreactivities are not altered at 2 and 5 days post-ischemia. Scale bars are 20 μm in F and M, valid for A–M. (G, N) Relative immunoreactivity (RI) as percent of SOD1 and SOD2 in the hippocampal CA1 pyramidal neurons in a 140 × 140 μm² (boxes) (n = 7 at each time point in each group, *P < 0.05, vs. the vehicle-sham group; †P < 0.05, vs. respective pre-time point group; #P < 0.05, vs. corresponding vehicle-ischemia group). Data in G and N are expressed as the mean ± SEM. SOD1: Cu/Zn superoxide dismutase; SOD2: Mn superoxide dismutase; QE: quercetin; SO: stratum oriens; SP: stratum pyramidale; SR: stratum radiatum.