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. 2017 Feb;12(2):267–275. doi: 10.4103/1673-5374.200810

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Apoptosis in L₆–S₁ DRG cells examined by terminal deoxyribonucleotidyl transferase (TdT)-mediated biotin-16-dUTP nick-end labeling (TUNEL) at 2 weeks after SCI.

(A) Cell nuclei of DRGs were very light brown, not much darker than the cytoplasm, in the sham group. (B) A large number of DRG apoptotic cell nuclei were stained dark brown in the SCI group. (C, D) Apoptotic DRG cell nuclei were stained brown or light brown in the TIIA and MP groups. (E) IOD of apoptotic DRG cells (n = 8 in each group; *P < 0.05). SCI: Spinal cord injury; TIIA: tanshinone IIA; MP: methylprednisolone; DRGs: dorsal root ganglions; IOD: integrated optical density.