Figure 4.

Serine 122 contributes to TFEB nuclear enrichment in the context of S211A mutation (A-C) Subcellular localization of transfected full-length-TFEB, S122A, S211A or S122A;S211A mutants in HeLa cells by biochemical fractionation (A) or confocal microscopy (B, C) following (or not) treatment with Torin1 (250 nM, 3 h). Graph represents average ± SD (n = 3; ≥ 100 cells per experiment; ***, P < 0.01). (D) YWHA protein immunoprecipitation from HeLa cells transfected with the indicated TFEB constructs. (E) Western blot for S211 phosphorylation of wild-type or mutant MYC-TFEB immunoprecipitated from transfected HeLa cells. (F) Proposed model for TFEB regulation by MTORC1. Scale bar: 20 μm.