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. 2017 Mar 14;8:333. doi: 10.3389/fpls.2017.00333

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Copyright © 2017 Sinha, Gupta and Senthil-Kumar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of microarray results by RT-qPCR. Transcript expression pattern of selected genes from microarray data was validated by RT-qPCR. Bar chart represents fold change expression (Log₂ FC) of the genes by RT-qPCR (light blue) and microarray (dark blue) under SD-combined stress. CaActin was used as reference gene for the qPCR normalization. Fold change was calculated over mock control. Each bar represents average of two biological and two technical replicates. Results presented are from one experiment.