FIGURE 5.

Overview of differential expression of xylem differentiation and secondary cell wall (SCW) related genes in SD- and LD-combined stress treatments. Diagrams represent vascular bundle structure with phloem, cambium and xylem vessel/fiber and xylem parenchyma. The order of the cell in the diagram is likely following the order present in dicot leaf where phloem is placed on abaxial followed by cambium and xylem on adaxial surface. DEGs with minimum fold change 1 (Log₂ converted) were identified in each individual and combined stressed transcriptome over their respective controls. DEGs were mapped onto ‘xylem development and SCW deposition’ pathways based on literature information. Major molecular events related to xylem differentiation and SCW synthesis in xylem vessel, tracheary element and fiber are represented once in the same box. The SCW synthesis pathway is representing only those genes which are differentially expressed in the SD-combined and LD-combined stress transcriptome. Thickening of SCW in xylem vessel and TE are represented as thick green wall of the cell. Increased cellulose and lignin deposition in LD-combined stress is represented as dark thick green color wall. Genes in red boxes represents up-regulated expression and in dark blue boxes represents down-regulated expression. Genes in the boxes with the light blue color has no differential expression. Details of the genes in the boxes are mentioned in the Supplementary File S2.