Table 2. Percentage of glomeruli with Col IV staining.
| Young | Aged | Fold increase and significance in aged mice compared to young mice | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| OC | JM | OC+JM | OC | JM | OC+JM | OC | JM | OC+JM | ||
| % glomeruli with no Col IV staining | Baseline | 83.75±4.63 | 69.17±11.51 | 77.50±6.42 | 42.62±10.83 | 29.68±10.38 | 38.07±10.48 | |||
| FSGS D28 | 47.67±24.92 | 20.61±12.19 | 36.07±17.68 | 35.52±16.94 | 12.22±11.92 | 23.87±13.69 | ||||
| % glomeruli with Col IV staining along BC only | Baseline | 15.00±4.23 | 29.58±12.14 | 21.25±6.54 | 49.36±10.99 | 55.77±13.10 | 51.37±10.94 | 3.29 fold p<0.0001 |
1.89 fold p<0.001 |
2.42 fold p<0.0001 |
| FSGS D28 | 25.84±10.98 | 23.64±10.80 | 24.90±7.89 | 43.67±12.49 | 43.61±11.05 | 43.65±10.37 | 1.69 fold p<0.01 |
1.84 fold p<0.001 |
1.75 fold p<0.0001 |
|
| Fold increase and significance in FSGS from baseline | 1.72 fold p<0.05 |
0.80 fold p=0.288 |
1.17 fold p=0.288 |
0.89 fold p=0.249 |
0.78 fold p<0.05 |
0.85 fold p=0.09 |
||||
| % glomeruli with Col IV staining in the tuft only | Baseline | 0.94±1.29 | 1.25±1.73 | 1.07±1.01 | 3.23±1.89 | 2.24±3.29 | 3.00±2.06 | 3.44 fold p<0.01 |
1.79 fold p=0.391 |
2.80 fold p<0.05 |
| FSGS D28 | 11.78±10.24 | 11.82±6.21 | 11.80±7.84 | 10.97±5.90 | 7.22±6.79 | 9.36±5.28 | 0.93 fold p=0.821 |
0.61 fold p=0.105 |
0.79 fold p=0.398 |
|
| Fold increase and significance in FSGS from baseline | 12.53 fold p<0.01 |
9.46 fold p<0.001 |
11.03 fold p<0.01 |
3.4 fold p<0.001 |
3.22 fold p<0.05 |
3.12 fold p<0.01 |
||||
| % glomeruli with Col IV staining along both BC and in the tuft | Baseline | 0.31±0.88 | 0.00±0.00 | 0.18±0.51 | 4.79±6.07 | 12.30±8.32 | 7.56±7.07 | 15.45 fold p<0.05 |
*young=0 p<0.001 |
42 fold p<0.01 |
| FSGS D28 | 14.71±10.73 | 43.94±14.21 | 27.23±9.70 | 12.84±8.18 | 36.94±14.17 | 23.13±9.50 | 0.87 fold p=0.645 |
0.84 fold p=0.251 |
0.85 fold p=0.318 |
|
| Fold increase and significance in FSGS from baseline | 47.45 fold p<0.01 |
*baseline=0 p<0.0001 |
151.28 fold p<0.0001 |
2.68 fold p<0.05 |
3.00 fold p<0.0001 |
3.06 fold p<0.001 |
||||
| % glomeruli with Col IV staining by combining BC only, tuft only, BC and tuft | Baseline | 16.25±4.63 | 30.83±11.51 | 22.50±6.42 | 57.38±10.83 | 70.32±10.38 | 61.93±10.48 | 3.53 fold p<0.0001 |
2.28 fold p<0.0001 |
2.75 fold p<0.0001 |
| FSGS D28 | 52.33±24.92 | 79.39±12.19 | 63.93±17.68 | 67.48±16.94 | 87.78±11.92 | 76.13±13.69 | 1.29 fold p=0.109 |
1.11 fold p=0.111 |
1.19 fold p=0.082 |
|
| Fold increase and significance in FSGS from baseline | 3.22 fold p<0.001 |
2.58 fold p<0.0001 |
2.84 fold p<0.0001 |
1.18 fold p=0.098 |
1.25 fold p<0.001 |
1.23 fold p<0.01 |
||||