Table 3.

Protocol for MGIT-DST of MDR-Mtb with DFO-B alone and in combination with INH and RIF

No	Drug	Initial Concentration of the drug preparation	Volume added to MGIT for Test	Final Concentration in MGIT tube except GC	No. of Middle Brook tubes
					GC	MDR
1	INH	8.3 μg/mL	100 μL	0.1 μg/mL	1	1
2	RIF	83 μg/mL	100 μL	1.0 μg/mL		1
3	DFO-B + INH	160 mg/mL + 8.3 μg/mL	12.5 μL + 100 μL	0.25 mg/mL and 0.1 μg/mL		1
4	DFO-B + INH	160 mg/mL + 8.3 μg/mL	25 μL and 100 μL	0.5 mg/mL and 0.1 μg/mL		1
5	DFO-B + INH	160 mg/mL + 8.3 μg/mL	50 μL and 100 μL	1.0 mg/mL and 0.1 μg/mL	1	1
6	DFO-B + RIF	160 mg/mL + 83 μg/mL	12.5 μL + 100 μL	0.25 mg/mL and 1.0 μg/mL		1
7	DFO-B + RIF	160 mg/mL + 83 μg/mL	25 μL + 100 μL	0.5 mg/mL and 1.0 μg/mL		1
8	DFO-B + RIF	160 mg/mL + 83 μg/mL	50 μL + 100 μL	1.0 mg/mL and 1.0 μg/mL		1
9	DFO-B	160 mg/mL	12.5 μL	0.25 mg/mL	1	1
10	DFO-B	160 mg/mL	25 μL	0.5 mg/mL		1
11	DFO-B	160 mg/mL	50 μL	1.0 mg/mL		1
12	DFO-B + FeCl3	160 mg/mL + 80 mg/mL	50 μL + 50 μL	1.0 mg/mL and 0.5 mg/mL		1