Table 3.
In vitro results of ROS, IL-8 mRNA expression and TEER measurement after exposure to CBNP
| A549 cells | 16HBE14o- cells | Calu-3 cells | |||||
|---|---|---|---|---|---|---|---|
| Relative DCF fluorescence | IL-8 | Relative DCF fluorescence | IL-8 | TEER (%) | IL-8 | ||
| P90 | 10 μg/ml | 1.2 ± 0.2 | 1.2 ± 0.4 | 1.5 ± 0.2** | 1.4 ± 0.2 | 103 ± 7 | 1.0 ± 0.2 |
| 50 μg/ml | 2.1 ± 0.5* | 2.1 ± 0.8** | 3.2 ± 1.1** | 1.7 ± 0.9 | 109 ± 8 | 1.1 ± 0.0 | |
| P90-BaP | 10 μg/ml | 1.1 ± 0.1 | 0.7 ± 0.2 | 1.3 ± 0.1 | 0.7 ± 0.3 | 97 ± 8 | 1.9 ± 0.6** |
| 50 μg/ml | 1.2 ± 0.1 | 0.7 ± 0.2 | 2.1 ± 0.1** | 1.9 ± 1.8 | 94 ± 9 | 3.0 ± 0.9** | |
| P90-9NA | 10 μg/ml | 0.9 ± 0.1 | 0.8 ± 0.2 | 1.1 ± 0.1 | 1.1 ± 0.5 | 105 ± 10 | 1.1 ± 0.1 |
| 50 μg/ml | 1.2 ± 0.2 | 0.8 ± 0.2 | 2.1 ± 0.3** | 2.0 ± 0.9* | 104 ± 9 | 1.2 ± 0.1 | |
| AS-PAH | 10 μg/ml | 2.3 ± 0.2** | 0.8 ± 0.3 | 1.8 ± 0.2** | 1.1 ± 0.4 | 91 ± 7* | 1.1 ± 0.2 |
| 50 μg/ml | 3.0 ± 0.4** | 0.8 ± 0.3 | 1.9 ± 0.1** | 1.4 ± 0.8 | 90 ± 6* | 2.1 ± 1.2** | |
Data show the n-fold change compared to medium controls of relative DCF fluorescence in A549 and 16HBE14o- cells, the n-fold change of relative IL-8 mRNA expression in A549, 16HBE14o- and Calu-3 cells, respectively, and the changes of TEER in Calu-3 cells after 24 h CBNP exposure
Data are mean ± SD. n = 3–5, *p < 0.05 and **p < 0.01 CBNP compared to medium controls analyzed by two-sided Student’s t-test for unpaired values (ROS analysis and TEER results) or Mann Whitney U test (mRNA expression data)