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. 2017 Mar 1;32(2):57–65. doi: 10.1089/cbr.2016.2141

FIG. 1.

FIG. 1.

Binding of PAN-622 conjugates to HAAH and cancer cells. (A) Binding of PAN-622-CHXA” conjugates to recombinant HAAH catalytic domain by ELISA; (B) binding of PAN-622-CHXA” conjugates to H460, human lung cancer cells as measured by cellular ELISA; (C) binding of PAN-622-DTPA-MAL conjugates to recombinant HAAH catalytic domain by ELISA; (D) binding of PAN-622-DTPA-MAL, 15 minutes, and PAN-622-CHXA” 10:1 conjugates to 4T1, mouse breast cancer cells as measured by cellular ELISA. mAU, milli absorbance units. ELISA, enzyme-linked immunosorbent assay; HAAH, human aspartyl (asparaginyl) β-hydroxylase.