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Binding and internalization of 111In-DTPA-pan622 antibody in 4T1 cells: (A) binding of 111In-DTPA-pan622 antibody to 4T1 cells. For control, 4T1 cells were preblocked with cold PAN-622. Another control was isotype-matching 111In-DTPA-2556 antibody to HIV-1 gp41; (B) internalization of 111In-DTPA-PAN-622 antibody into 4T1 cells. The percentage of radioactivity remaining in the cells after the acid wash is shown.
