4T1 tumor-bearing balb/c mice injected with 111In-DTPA-PAN-622 antibody and imaged on IVIS Imager for Cherenkov luminescence at 24 and 48 hours postinjection: (A) 24 hours, mice in prone position; (B) 24 hours, mice in supine position; (C) 48 hours, mice in prone position; (D) 48 hours, mice in supine position. The suggested location of the lung metastases (A, C) and primary tumors in the mammary fat pad (B, D) is shown with red arrows.