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. 2017 Mar 21;17:4. doi: 10.1186/s12899-017-0031-x

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overfeeding of adult zebrafish with either NFD or HFD leads to an obese phenotype. a Schematic overview of the 8-week feeding protocol and phenotyping of zebrafish. b Exemplary images of zebrafish included in each of the analyzed dietary groups at week 8 of feeding. Black arrows point to the abdominal region. Overfeeding of zebrafish with either NFD or HFD resulted in a significant increase in weight (c), standard length (d), BMI (e), and Fulton’s condition index (f) compared to control zebrafish. Statistical analyses were performed using Two-Way ANOVA and Bonferroni post-test and significant p-values are indicated. *, p < 0.05; **, p < 0.01; ***, p < 0.001; NFD, normal fat diet; HFD, high-fat-diet; OF, overfeeding; BMI, body mass index; MRI, Magnetic Resonance Imaging; AT, adipose tissue