Fig. 1.

Unwashed PSEC or washed PSEC was loaded onto CBM and membrane morphology and cell attachment was evaluated. Platelet concentrations were divided into two equivalent aliquots and processed as described to generate unwashed PSEC and washed PSEC (a). CBM were soaked with unwashed PSEC or washed PSEC, immediately frozen and lyophilized. Morphology of CBM loaded with unwashed PSEC or washed PSEC was evaluated with scanning electron microscopy and cell attachment was assessed with fluorescence microscopy (b). For scanning electron microscopy images were taken at a 500-fold magnification (The white bar represents 200 μm) (c). An in vitro bioassay was used to reveal the attachment kinetics of GF and PDLF to CBM: PDLF (d) and GF (e) were pre-labeled with DiI and seeded on CBM loaded with unwashed PSEC or washed PSEC. The membranes were washed and images were taken 1, 3, and 24 h and the attached cells were counted. The data points show the mean ± standard deviation relative to the untreated control (n = 3). The dashed line represents the levels of the untreated control