Fig. 2.

Mitogenic capacity of supernatants from CBM loaded with unwashed PSEC and washed PSEC. An in vitro bioassay was used to reveal the release kinetics of platelet-released supernatants of CBM: Supernatants of CBM loaded with unwashed PSEC and washed PSEC were collected at hour 1, 3, 6, 24, and 48 (a). The supernatants were subjected to cell cultures where their capacity to stimulate proliferation was assessed by ³[H]thymidine incorporation into GF (b) and PDLF (c). The data points show the mean ± standard deviation relative to the control CBM. For proliferation of GF n = 10 and for proliferation of PDLF n = 12. The dashed line represents the levels of the control CBM. The bar diagrams represent the AUC. * p < 0.05