Fig. 4.

A high-throughput genotype and statistical power analysis of wheat dark respiration (R _dark) of the youngest fully expanded leaf. a Leaf respiration in the dark (R _dark) across 138 wheat cultivars grown under common conditions in a controlled environment growth room and measured at 21.5 ± 1 °C. The experiment was replicated on four occasions and the means (green columns) ± SE (black bars) of four biological replicates are presented. b Statistical power analysis of the wheat dataset with lines representing different numbers of biological replicates (3, 4, 6, or 10) plotted as a function of statistical power versus difference in genotype mean values. c Statistical power analysis of the wheat dataset with lines representing differences in mean values between genotypes equal to 20, 40 or 60% of the overall mean (equal to effect sizes of 1, 2 and 3 times the standard deviation) plotted as a function of statistical power versus biological replicates