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. 2017 Mar 16;19(4):321–332. doi: 10.1016/j.neo.2017.02.001

Figure 4.

Figure 4

3′-untranslated regions (UTR) of UBQLN1 and UBQLN2 are regulated by MIR155. Dual luciferase assay in A549 and 293 T cells transfected with luciferase reporter plasmids pEZX-MT01 (empty vector; EV), pEZX-UBQLN1 (contains 3′-UTR of UBQLN1; 3′-UTR) or pEZX-UBQLN2 (contains 3′-UTR of UBQLN2; 3′-UTR) and MIR155 mimic (155 m) or MIR155 inhibitor (155i) or NTC. Relative luciferase activity was calculated by normalizing firefly luciferase activity with Renilla luciferase activity (transfection control). Relative luciferase activities of cells transfected with 3′-UTR plasmid and NTC is considered as 1 (equals to 100%) and other activities are presented in comparison to them.