Figure 6.

Expression of MIR155 induced clonogenic capabilities and proliferation of lung carcinoma cells. A) Cells are transfected with MIR155m or MIR155i or NTC or KIff11 (positive control). After 24 hours of transfection, each well of 6-well dish was plated with 1000 cells and grown for 10 day. After 10 days, cells were fixed, stained and destained as described in methodology. Bar diagram represents average number of colonies counted after staining. B) Alamar blue assay in A549 and H358 cells transfected with MIR155m or MIR155i or Kiff11 or NTC. After 24 hours of transfection, 96 well plate were seeded with 1000 cells/well and Alamar blue assay was carried out on 0 day (day of cell seeding), and next 4 consecutive days. Day 0 was used to normalize the counts of next 4 days for any possible difference in cell seeding. Relative activities were then calculated each day against NTC (considered as 1).